A few months ago we were treated to a mysterious teaser for a new film titled Despicable Me, which had a cornucopia of comedic names attached to it like Steve Carell, Jason Segel, Will Arnett, Kristin Wiig, Russel Brand, and Danny McBride. We’re finally getting a bit more of the puzzle solved as the second trailer for the film has hit the web.

The film focuses on Gru (Carell), the world’s #2 super villain, who is constantly at the throat of #1, his competition and enemy, Vector (Segel). The film takes a few interesting turns, which force Gru to rethink his evil ways.

The film comes from the studio and animation department that brought us Ice Age, Robots, and Horton Hears a Who! and the writing team who brough us Bubble Boy and The Santa Clause 2. Check out the second teaser after the jump, which focuses on the rivalry between Gru and Vector.

Despicable Me hits theaters on July 9th, 2010.